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Nicastrin Polyklonaler Antikörper

Nicastrin Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14071-1-PBS

Synonyme

UNQ1874/PRO4317, NCSTN, KIAA0253

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using Nicastrin Polyclonal antibody (14071-1-AP)

    WB analysis of mouse brain using 14071-1-AP (same clone as 14071-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14071-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using Nicastrin Polyclonal antibody (14071-1-AP)

    WB analysis of C6 using 14071-1-AP (same clone as 14071-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14071-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Nicastrin Polyclonal antibody (14071-1-AP)

    WB analysis of human brain using 14071-1-AP (same clone as 14071-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14071-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using Nicastrin Polyclonal antibody (14071-1-AP)

    IF Staining of C6 using 14071-1-AP (same clone as 14071-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using Nicastrin antibody (14071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14071-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14071-1-PBS bindet in WB, IF/ICC, Indirect ELISA Nicastrin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Nicastrin fusion protein Ag5137
Vollständiger Name nicastrin
Berechnetes Molekulargewicht 709 aa, 78.4 kDa
Beobachtetes Molekulargewicht 110 kDa
GenBank-ZugangsnummerBC047621
Gene symbol Nicastrin
Gene ID (NCBI) 23385
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease cleaves many type I membrane proteins including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytical processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).