Ninein Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Ninein Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1B10A8

Rezensionen (2)

Kat-Nr. : 67132-1-Ig

Synonyme

NIN, 1B10A8, Glycogen synthase kinase 3 beta-interacting protein, GSK3B interacting protein, GSK3B-interacting protein

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  WB analysis using 67132-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 67132-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HT-29-Zellen, MCF-7-Zellen, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

67132-1-Ig bindet in WB, IHC, IF/ICC, ELISA Ninein und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ninein fusion protein Ag14652
• Vollständiger Name: ninein (GSK3B interacting protein)
• Berechnetes Molekulargewicht: 2090 aa, 243 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC034708
• Gene symbol: Ninein
• Gene ID (NCBI): 51199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.

Publikationen

Species	Application	Title
human	WB,IF	Dev Cell; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method; Authors - Sarah Carden ; KO Validated
human	IF	J Cell Sci; Roles for CEP170 in cilia function and dynein-2 assembly; Authors - Johannes F Weijman
	IF	Elife; Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development; Authors - Cayla E Jewett

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (07-12-2021)

This antibody stains well ninein pool at the centrosome, in MetOH fixation conditions

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:300 IF 1:500WB
• Cell Tissue Type: HAP1, H9, RPE1 and HEK293T cells

FH

Sarah (Verified Customer) (07-06-2020)

Antibody used at 1:1000 to visualise ninein protein (from 20ug cell lysate) in Hap1 control and Ninein KO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hap1

Ninein Antibody Western Blot validation (1:1000 dilution) in Hap1 (Cat no:67132-1-Ig)