Ninein Monoklonaler Antikörper

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• KD/KO Validated

Ninein Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B10A8

Kat-Nr. : 67132-1-PBS

Synonyme

NIN, 1B10A8, Glycogen synthase kinase 3 beta-interacting protein, GSK3B interacting protein, GSK3B-interacting protein

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  WB analysis using 67132-1-Ig (same clone as 67132-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67132-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Ninein und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ninein fusion protein Ag14652
• Vollständiger Name: ninein (GSK3B interacting protein)
• Berechnetes Molekulargewicht: 2090 aa, 243 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC034708
• Gene symbol: Ninein
• Gene ID (NCBI): 51199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.