NLRP3 Polyklonaler Antikörper

NLRP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(43)

Kat-Nr. : 30109-1-AP

Synonyme

AGTAVPRL, AII/AVP, Mmig1, MWS, NALP3

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  WB analysis using 30109-1-AP

  Non-treated RAW 264.7 cells and LPS-treated RAW 264.7 cells lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (NLRP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (NLRP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (NLRP3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 30109-1-AP

  Rat spleen tissue was subjected to SDS PAGE, followed by western blot with 30109-1-AP (NLRP3 antibody) at a dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 30109-1-AP

  IP result of anti-NLRP3 (IP:30109-1-AP, 4ug; Detection:30109-1-AP 1:5000) with RAW 264.7 cells lysate 1680 ug.

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  IP experiment of RAW 264.7 using 30109-1-AP

  IP result of anti-Nlrp3 (IP:30109-1-AP, 4ug; Detection:30109-1-AP 1:4000) with RAW 264.7 cells lysate 1200 ug.

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  IHC staining of mouse liver using 30109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30109-1-AP (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Nlrp3 antibody (30109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of RAW 264.7 using 30109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using NLRP3 antibody (30109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS behandelte RAW 264.7-Zellen, Rattenmilzgewebe, RAW 264.7-Zellen
• Erfolgreiche IP: RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 37 publications below
• IHC: See 8 publications below
• IF: See 12 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

30109-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA NLRP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NLRP3 fusion protein Ag32515
• Vollständiger Name: NLR family, pyrin domain containing 3
• Berechnetes Molekulargewicht: 118kd
• Beobachtetes Molekulargewicht: 108-120 kDa
• GenBank-Zugangsnummer: NM_145827
• Gene symbol: Nlrp3
• Gene ID (NCBI): 216799
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse NLRP3.

Publikationen

Species	Application	Title
mouse	IHC	Phytomedicine; ErTao decoction alleviates liver fibrosis by suppressing STING-mediated macrophages and NLRP3 inflammasome activation; Authors - Hui Jia
mouse	WB	J Agric Food Chem; Probiotic Combination of Limosilactobacillus fermentum HF07 and Lactococcus lactis HF08 Targeting Gut Microbiota-Secondary Bile Acid Metabolism Ameliorates Inflammation and Intestinal Barrier Dysfunction in Aging Colitis; Authors - Xiaolin Liu
mouse	WB	J Agric Food Chem; Ocotillol-Type Pseudoginsenoside-F11 Alleviates Lipopolysaccharide-Induced Acute Kidney Injury through Regulation of Macrophage Function by Suppressing the NF-κB/NLRP3/IL-1β Signaling Pathway; Authors - Yaru Wang
mouse	WB,IF	Int Immunopharmacol; Triggering receptor expressed on myeloid cells-1 aggravates obliterative bronchiolitis via enhancing the proinflammatory phenotype of macrophages; Authors - Junhao Wan
mouse	WB	Int Immunopharmacol; UCP2 knockout exacerbates sepsis-induced intestinal injury by promoting NLRP3-mediated pyroptosis; Authors - Bolun Huang
rat,mouse	WB,IHC,IF	Int Immunopharmacol; Therapeutic potential of quercitrin in intervertebral disc degeneration: Targeting pyroptosis and inflammation; Authors - Xinyu Wu