NOV/CCN3 Rekombinanter Antikörper

NOV/CCN3 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250054B2

Kat-Nr. : 85731-1-PBS

Synonyme

CCN family member 3, Ccn3, Cellular communication network factor 3, Nephroblastoma-overexpressed gene protein homolog, Nov

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  WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

  Mouse brain tissue was subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02088-1

  Cytometric bead array standard curve of MP02088-1, MOUSE Nov/CCN3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85731-2-PBS. Detection antibody: 85731-1-PBS. Standard: Eg2856. Range: 1.563-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

85731-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA NOV/CCN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NOV/CCN3 fusion protein Eg2856
• Vollständiger Name: nephroblastoma overexpressed gene
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: NM_010930.4
• Gene symbol: Nov
• Gene ID (NCBI): 18133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCN family member 3 (CCN3), also known as Nephroblastoma overexpressed (NOV) protein, is a member of the CCN protein family (PMID: 1309586). CCN3 is predominantly expressed in the cytoplasm, nucleus, and extracellular space. As a subset of extracellular matrix proteins, it can directly bind to the integrin receptor family on the cell surface and participate in signal transduction between the cell surface and the extracellular matrix structure (PMID: 37378812). CCN3 exhibits anti-proliferative activity in normal cells and most tumor cells (PMID: 17340618). CCN3 co-localizes with connexin 43 (C×43) in the plasma membrane, and these two proteins physically interact to inhibit cell growth (PMID: 15213231). CCN3 can induce myofibroblast differentiation through the Notch pathway (PMID: 12050162). CCN can promote adhesion of endothelial cells, vascular smooth muscle cells, and fibroblasts through heparan sulfate proteoglycans (HSPG) (PMID: 30158025).