Nucleoside phosphorylase Rekombinanter Antikörper

Nucleoside phosphorylase Rekombinant Antikörper für WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251603E11

Kat-Nr. : 86651-1-PBS

Synonyme

PNP, EC:2.4.2.1, Inosine phosphorylase, Inosine-guanosine phosphorylase, PRO1837

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  WB analysis using 86651-1-RR (same clone as 86651-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86651-1-RR (Nucleoside phosphorylase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86651-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02719-1

  The mean Nucleoside phosphorylase concentration was determined to be 313.73 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA sample test of MP02719-1

  Plasma of six individual healthy human donors was measured. The Nucleoside phosphorylase concentration of detected samples was determined to be 38.13 ng/mL with a range of 18.89-62.76 ng/mL.

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  Sandwich ELISA standard curve of MP02719-1

  Sandwich ELISA standard curve of MP02719-1, Human Nucleoside phosphorylase Recombinant Matched Antibody Pair - PBS only. 86651-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12507. 86651-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  IF Staining of NIH/3T3 using 86651-1-RR (same clone as 86651-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using NP antibody (86651-1-RR, Clone: 251603E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86651-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86651-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86651-1-RR against Human Nucleoside phosphorylase were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86651-1-PBS bindet in WB, IF/ICC, Sandwich ELISA, Indirect ELISA Nucleoside phosphorylase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Nucleoside phosphorylase fusion protein Ag12507
• Vollständiger Name: nucleoside phosphorylase
• Berechnetes Molekulargewicht: 289 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC106074
• Gene symbol: Purine nucleoside phosphorylase
• Gene ID (NCBI): 4860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Purine nucleoside phosphorylases (PNP) is a ubiquitous enzyme of purine metabolism that functions in the salvage pathway, including even those of protozoan parasites, thus enabling the cells to utilize purine bases recovered from metabolized purine ribo- and deoxyribonucleosides to synthesize purine nucleotides (PMID: 11337031, 23332162). PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function. hPNP is widely distributed in various tissues and cells of the human body, with the highest activity in kidney, peripheral lymphocytes and granulocytes (PMID: 38701712).