Nurr1/NR4A2 Polyklonaler Antikörper

Nurr1/NR4A2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10975-2-PBS

Synonyme

NR4A2, NURR1, HZF 3, Immediate-early response protein NOT, NOT

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  WB analysis of SH-SY5Y using 10975-2-AP (same clone as 10975-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 10975-2-AP (same clone as 10975-2-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  IF Staining of Customer Sample using 10975-2-AP (same clone as 10975-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded Customer Sample customer using Nurr1/NR4A2 antibody (10975-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10975-2-AP (same clone as 10975-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Nurr1/NR4A2 antibody (10975-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10975-2-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA Nurr1/NR4A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nurr1/NR4A2 fusion protein Ag1418
• Vollständiger Name: nuclear receptor subfamily 4, group A, member 2
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC009288
• Gene symbol: Nurr1
• Gene ID (NCBI): 4929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].