Nurr1/NR4A2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Nurr1/NR4A2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H5B4

Kat-Nr. : 66878-1-PBS

Synonyme

NR4A2, NURR1, 1H5B4, HZF 3, Immediate-early response protein NOT

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  WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66878-1-Ig (same clone as 66878-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66878-1-Ig (same clone as 66878-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66878-1-Ig (same clone as 66878-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66878-1-PBS bindet in WB, IHC, Indirect ELISA Nurr1/NR4A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Nurr1/NR4A2 fusion protein Ag1418
• Vollständiger Name: nuclear receptor subfamily 4, group A, member 2
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC009288
• Gene symbol: Nurr1
• Gene ID (NCBI): 4929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].