O-GlcNAc Monoklonaler Antikörper

O-GlcNAc Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

n/a

Anwendung

WB

Konjugation

Unkonjugiert

CloneNo.

CTD110.6

Kat-Nr. : 65292-1-Ig

Synonyme

glycosylation, O Glc, O-Glc, O-glycosylation, O-linked GlcNAc

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  WB analysis using 65292-1-Ig

  Untreated or PUGNAc (an inhibitor of N-acetyl-β-D-glucosaminidase) treated HeLa cells were subjected to SDS PAGE followed by western blot with 65292-1-Ig (O-GlcNAc antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65292-1-Ig bindet in WB O-GlcNAc und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: O-GlcNAc
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

O-GlcNAc (O-linked β-N-acetylgulcosamine) is a post-translational modification of serine and threonine residues in proteins. O-GlcNAc modification is abundant in all multicellular eukaryotes, exclusively found on nuclear and cytoplasmic proteins rather than membrane proteins and secretory proteins (PMID: 11269319; 10924527). O-GlcNAc is attached to the protein backbone by enzymatic addition of the N-acetylgulcosamine (GlcNAc) moiety of uridine 5′-diphospho (UDP)-GlcNAc to the hydroxyl oxygen of serines or threonines by the O-linked β-N-acetylgulcosamine transferase (OGT). O-GlcNAc glycosylated proteins can be reversiblely deglycosylated by β-D-N-acetylgulcosaminase (O-GlcNAcase) (PMID: 21526146). O-GlcNAc modification couples many biological processes and plays important roles in development, normal physiology and physiopathology (PMID: 32349769).