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O-GlcNAc Monoklonaler Antikörper

O-GlcNAc Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

n/a

Anwendung

WB

Konjugation

Unkonjugiert

CloneNo.

CTD110.6

Kat-Nr. : 65292-1-PBS

Synonyme

glycosylation, O Glc, O-Glc, O-glycosylation, O-linked GlcNAc

Filter:
  • Western Blot (WB) analysis of various lysates using O-GlcNAc Monoclonal antibody (65292-1-Ig)

    WB analysis using 65292-1-Ig (same clone as 65292-1-PBS)

    Untreated or PUGNAc (an inhibitor of N-acetyl-β-D-glucosaminidase) treated HeLa cells were subjected to SDS PAGE followed by western blot with 65292-1-Ig (O-GlcNAc antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 65292-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

65292-1-PBS bindet in WB O-GlcNAc und zeigt Reaktivität mit n/a

Getestete Reaktivität n/a
Wirt / Isotyp Maus / IgM
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name O-GlcNAc
Gene symbol
Gene ID (NCBI)
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Thiophiler Affinitätschromatograph
Lagerungspuffer PBS Only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

O-GlcNAc (O-linked β-N-acetylgulcosamine) is a post-translational modification of serine and threonine residues in proteins. O-GlcNAc modification is abundant in all multicellular eukaryotes, exclusively found on nuclear and cytoplasmic proteins rather than membrane proteins and secretory proteins (PMID: 11269319; 10924527). O-GlcNAc is attached to the protein backbone by enzymatic addition of the N-acetylgulcosamine (GlcNAc) moiety of uridine 5′-diphospho (UDP)-GlcNAc to the hydroxyl oxygen of serines or threonines by the O-linked β-N-acetylgulcosamine transferase (OGT). O-GlcNAc glycosylated proteins can be reversiblely deglycosylated by β-D-N-acetylgulcosaminase (O-GlcNAcase) (PMID: 21526146). O-GlcNAc modification couples many biological processes and plays important roles in development, normal physiology and physiopathology (PMID: 32349769).