OAS1/3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OAS1/3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Rezensionen (1)

Kat-Nr. : 14955-1-AP

Synonyme

(2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OAS1/3, OIAS, OIASI, p46/p42 OAS

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Galerie der Validierungsdaten

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  WB analysis using 14955-1-AP

  Untreated and IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 14955-1-AP (OAS1/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14955-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14955-1-AP (OAS1/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14955-1-AP (OAS1/3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14955-1-AP (OAS1/3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14955-1-AP (OAS1/3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14955-1-AP (OAS1/3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14955-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14955-1-AP (OAS1/3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: IFN alpha treated HeLa cells, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

14955-1-AP bindet in WB, IHC, IF, CoIP, ELISA OAS1/3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OAS1/3 fusion protein Ag6793
• Vollständiger Name: 2',5'-oligoadenylate synthetase 1, 40/46kDa
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: ~40 kDa, 100-120 kDa
• GenBank-Zugangsnummer: BC000562
• Gene symbol: OAS1
• Gene ID (NCBI): 4938
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009). This antibody may have cross-reaction with OAS3 protein due to the high homology.

Publikationen

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen ; KO Validated
human		Adv Sci (Weinh); The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.; Authors - Qixue Wang
human	WB	PLoS Biol; Resurrection of 2'-5'-oligoadenylate synthetase 1 (OAS1) from the ancestor of modern horseshoe bats blocks SARS-CoV-2 replication; Authors - Spyros Lytras
mouse	WB	Autophagy; USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.; Authors - Xibao Zhao
human	WB	J Invest Dermatol; OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.; Authors - Yan-Zhou Huang ; KD Validated
human	WB,IHC	Elife; A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.; Authors - Jia Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Seong (Verified Customer) (11-27-2018)

OAS1 polyclonal antibody (14955-1-AP) did work by Western Blot (1:1,500) of ARPE-19 cells induced with interferon gamma but the band was very weak in uninduced cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,500
• Cell Tissue Type: human ARPE-19 cells