OAS2 Polyklonaler Antikörper

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• KD/KO Validated

OAS2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Kat-Nr. : 19279-1-AP

Synonyme

(2-5')oligo(A) synthase 2, 2-5A synthase 2, 2'-5'-oligoadenylate synthase 2, EC:2.7.7.84

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  WB analysis using 19279-1-AP

  IFN alpha treated A549 cells were subjected to SDS PAGE followed by western blot with 19279-1-AP (OAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19279-1-AP (OAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19279-1-AP

  IP result of anti-OAS2 (IP:19279-1-AP, 4ug; Detection:19279-1-AP 1:500) with Jurkat cells lysate 2280 ug.

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  IHC staining of human colon cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 19279-1-AP (OAS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19279-1-AP (OAS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19279-1-AP (OAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19279-1-AP (OAS2 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19279-1-AP (OAS2 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 19279-1-AP (OAS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: IFN alpha treated A549 cells, Daudi-Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: human colon cancer, humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Produktinformation

19279-1-AP bindet in WB, IHC, IF, IP, ELISA OAS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OAS2 fusion protein Ag6170
• Vollständiger Name: 2'-5'-oligoadenylate synthetase 2, 69/71kDa
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 66-71 kDa
• GenBank-Zugangsnummer: BC049215
• Gene symbol: OAS2
• Gene ID (NCBI): 4939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are IFN-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS / p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody is specific to isoform p71 and isoform p69 of OAS2.

Publikationen

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen
human	WB	Nat Cell Biol; Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes; Authors - Dario F De Jesus
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
human	IF	Sci Adv; IL-27 signaling activates skin cells to induce innate antiviral proteins and protects against Zika virus infection.; Authors - Jeffery T Kwock
human	WB	Sci Immunol; OAS1/RNase L executes RIG-I ligand-dependent tumor cell apoptosis.; Authors - Daniel F R Boehmer
mouse,human	IF	J Invest Dermatol; Skin Injury Activates A Rapid TRPV1-dependent Antiviral Protein Response.; Authors - Vivian Lei