OAS3 Polyklonaler Antikörper

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• KD/KO Validated

OAS3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 21915-1-AP

Synonyme

(2 5)oligo(A) synthase 3, 2 5 oligoadenylate synthase 3, 2 5A synthase 3, OAS3, p100, p100 OAS, p100OAS

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Galerie der Validierungsdaten

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  WB analysis using 21915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21915-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 21915-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 21915-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21915-1-AP

  Immunofluorescent analysis of HeLa cells using 21915-1-AP (OAS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Produktinformation

21915-1-AP bindet in WB, IHC, IF, ELISA OAS3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OAS3 fusion protein Ag16150
• Vollständiger Name: 2'-5'-oligoadenylate synthetase 3, 100kDa
• Berechnetes Molekulargewicht: 1087 aa, 121 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC113746
• Gene symbol: OAS3
• Gene ID (NCBI): 4940
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS3, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). It is also named as p100 OAS and belongs to the 2-5A synthase family. OAS3 is involved in inhibition of cellular protein synthesis and in viral infection resistance. This antibody may have weak cross-reaction with OAS1 protein due to the highly homology.

Publikationen

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen ; KO Validated
human		Nat Commun; Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.; Authors - Wei Liu
human	WB	J Invest Dermatol; OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.; Authors - Yan-Zhou Huang ; KD Validated
human	WB	Virol Sin; EV71 3C protease cleaves host anti-viral factor OAS3 and enhances virus replication.; Authors - Xiaolei Zhou ; KD Validated
human	WB	Virol Sin; IFN-β1b induces OAS3 to inhibit EV71 via IFN-β1b/JAK/STAT1 pathway; Authors - Baisong Zheng
human	IHC	Am J Cancer Res; Identification of potential immune-related circRNA-miRNA-mRNA regulatory network in cutaneous squamous cell carcinoma; Authors - Yuanfeng Wei