OAT1 Polyklonaler Antikörper

OAT1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 26574-1-AP

Synonyme

HOAT1, hPAHT, hROAT1, OAT1, Organic anion transporter 1, PAH transporter, PAHT, ROAT1, SLC22A6

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 26574-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26574-1-AP (OAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26574-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26574-1-AP (OAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26574-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26574-1-AP (OAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IF: See 1 publications below

Produktinformation

26574-1-AP bindet in WB, IHC, IF, ELISA OAT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OAT1 fusion protein Ag24234
• Vollständiger Name: solute carrier family 22 (organic anion transporter), member 6
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 60-65 and 70-80 kDa
• GenBank-Zugangsnummer: BC033682
• Gene symbol: SLC22A6
• Gene ID (NCBI): 9356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OAT1, also named as SLC22A6, has two GXXXG motifs in its transmembrane which is associated with protein processing and oligomerization of other proteins. OAT1 is involved in the renal elimination of endogenous and exogenous organic anions. It has been reported that OAT1 plays a key role in clearing endogenous metabolites, toxins and drugs from blood. OAT1 has some isoforms and the calculated MW ranges from 55 kDa to 62 kDa. 26574-1-AP antibody detects the 60-65 kDa (native forms) and 70-80 kDa (mature forms) protein in SDS-PAGE. (PMID: 21340049, 23389457, 23196129)

Publikationen

Species	Application	Title
mouse	WB	Phytomedicine; Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice; Authors - Xiao-Li Guo
mouse	WB	J Ethnopharmacol; Fuling-Zexie formula attenuates hyperuricemia-induced nephropathy and inhibits JAK2/STAT3 signaling and NLRP3 inflammasome activation in mice; Authors - Meixi Lu
mouse	WB	J Ethnopharmacol; Simiao San alleviates hyperuricemia and kidney inflammation by inhibiting NLRP3 inflammasome and JAK2/STAT3 signaling in hyperuricemia mice; Authors - Yueyi Zhang
mouse	WB	Am J Physiol Renal Physiol; SGLT2 inhibitor dapagliflozin protects the kidney in a murine model of Balkan nephropathy; Authors - Yuji Oe
human	WB	Tissue Eng Part A; Inhibition of TGF-β improves primary renal tubule cell differentiation in long-term culture; Authors - Kuniko Hunter
mouse	WB	Eur J Pharmacol; Berberrubine attenuates potassium oxonate- and hypoxanthine-induced hyperuricemia by regulating urate transporters and JAK2/STAT3 signaling pathway; Authors - Guoshu Lin