OCIAD1 Polyklonaler Antikörper

OCIAD1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16634-1-AP

Synonyme

TPA018, Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA

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  WB analysis using 16634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16634-1-AP (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16634-1-AP

  IP result of anti-OCIAD1 (IP:16634-1-AP, 4ug; Detection:16634-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of human liver cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16634-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCIAD1 antibody (16634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 16634-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OCIAD1 antibody (16634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe, humanes Pankreaskarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16634-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA OCIAD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OCIAD1 fusion protein Ag9977
• Vollständiger Name: OCIA domain containing 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 29-35 kDa
• GenBank-Zugangsnummer: BC003409
• Gene symbol: OCIAD1
• Gene ID (NCBI): 54940
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).

Publikationen

Species	Application	Title
human	WB,IF	PLoS One; OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease.; Authors - Huong T L Tran
human	IHC	Pathol Int; Increased expression of OCIA domain containing 2 during stepwise progression of ovarian mucinous tumor.; Authors - Nagata Chigusa C