OCIAD1 Monoklonaler Antikörper

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• KD/KO Validated

OCIAD1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C10C3

Kat-Nr. : 66698-1-PBS

Synonyme

Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ

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  WB analysis of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 66698-1-Ig (same clone as 66698-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 66698-1-Ig (same clone as 66698-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50614-1

  Cytometric bead array standard curve of MP50614-1, OCIAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66698-1-PBS. Detection antibody: 66698-2-PBS. Standard:Ag9977. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP50614-2

  Cytometric bead array standard curve of MP50614-2, OCIAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66698-1-PBS. Detection antibody: 66698-3-PBS. Standard:Ag9977. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66698-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA OCIAD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OCIAD1 fusion protein Ag9977
• Vollständiger Name: OCIA domain containing 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC003409
• Gene symbol: OCIAD1
• Gene ID (NCBI): 54940
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).