OCRL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OCRL Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17695-1-AP

Synonyme

INPP5F, LOCR, OCRL, OCRL1

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 17695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17695-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17695-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17695-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17695-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17695-1-AP

  IP Result of anti-OCRL (IP:17695-1-AP, 4ug; Detection:17695-1-AP 1:300) with HeLa cells lysate 1080ug.

• 

  IHC staining of mouse kidney using 17695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17695-1-AP (OCRL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17695-1-AP (OCRL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17695-1-AP bindet in WB, IP, IHC, IF, ELISA OCRL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OCRL fusion protein Ag11900
• Vollständiger Name: oculocerebrorenal syndrome of Lowe
• Berechnetes Molekulargewicht: 893 aa, 103 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC094726
• Gene symbol: OCRL
• Gene ID (NCBI): 4952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OCRL is also named as INPP5F, OCRL1 and belongs to the 5-phosphatase gene family and that Lowe syndrome represents an inborn error of inositol phosphate metabolism(PMID: 9430698).The protein product of the gene that when mutated is responsible for Lowe syndrome, or oculocerebrorenal syndrome (OCRL), is an inositol polyphosphate 5-phosphatase.It may function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes.It has 2 isoforms produced by alternative splicing.Defects in OCRL are the cause of Lowe oculocerebrorenal syndrome (OCRL) and Dent disease type 2 (DD2).This antibody is specific to OCRL.

Publikationen

Species	Application	Title
human	WB,IF	Mol Autism; Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells.; Authors - Jesse Barnes ; KO Validated
human	WB	Nephrol Dial Transplant; Identification of novel OCRL isoforms associated with phenotypic differences between Dent disease-2 and Lowe syndrome.; Authors - Nana Sakakibara
human	WB	J Neurodev Disord; Transcriptome analysis of neural progenitor cells derived from Lowe syndrome induced pluripotent stem cells: identification of candidate genes for the neurodevelopmental and eye manifestations.; Authors - Hequn Liu ; KD Validated
human	IHC	BMC Med Genomics; Novel pathogenic OCRL mutations and genotype-phenotype analysis of Chinese children affected by oculocerebrorenal syndrome: two cases and a literature review; Authors - Yu Zhang
mouse	WB	Cell Death Dis; Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.; Authors - Viktoriya Zhuravleva ; KD Validated