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OCT4/POU5F1 Polyklonaler Antikörper

OCT4/POU5F1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(283)

Kat-Nr. : 11263-1-AP

Synonyme

OCT4, POU5F1, OCT3, Oct-3, Oct-4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, Maushirngewebe, Mausembryogewebe, NCCIT-Zellen, Rattenhirngewebe, U-251-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11263-1-AP bindet in WB, IP, ELISA OCT4/POU5F1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen OCT4/POU5F1 fusion protein Ag1794
Vollständiger Name POU class 5 homeobox 1
Berechnetes Molekulargewicht 39 kDa
Beobachtetes Molekulargewicht38 kDa, 50-60 kDa
GenBank-ZugangsnummerBC020712
Gene symbol POU5F1
Gene ID (NCBI) 5460
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

 What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

 What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

 What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).




Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for OCT4/POU5F1 antibody 11263-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cell

Temporal proteomics during neurogenesis reveals large-scale proteome and organelle remodeling via selective autophagy

Authors - Alban Ordureau
humanWB

Cell Rep Med

CD97 maintains tumorigenicity of glioblastoma stem cells via mTORC2 signaling and is targeted by CAR Th9 cells

Authors - Shuchang Zhou
mouse

Bone Res

Type II collagen-positive progenitors are important stem cells in controlling skeletal development and vascular formation.

Authors - Xinhua Li
humanWB

J Exp Clin Cancer Res

Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma

Authors - Yongzhi Zhao
mouse

J Control Release

Dual-phase nanoscissors disrupt vasculature-breast cancer stem cell crosstalk for breast cancer treatment

Authors - Yao Qi
humanWB

J Pineal Res

Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis

Authors - Xinyue Luo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Zhiping (Verified Customer) (06-21-2023)

This antibody works well for WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Udesh (Verified Customer) (11-18-2022)

Worked well in Osteosarcoma U2OS cells. 20ug protein loaded. Dilution 1:2000 in 5% NFDM.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS cells
OCT4/POU5F1 Antibody Western Blot validation (1:2000 dilution) in U2OS cells (Cat no:11263-1-AP)
FH

Gongsheng (Verified Customer) (11-02-2022)

This antibody works well in WB, diluation 1:1000, incubation at +4 °C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
FH

Silvia (Verified Customer) (10-19-2021)

It works well for Immunofluorescence on hiPSCs

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500