OCT4 Monoklonaler Antikörper

OCT4 Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB

Konjugation

HRP Fluorescent Dye

CloneNo.

1C4B6

Kat-Nr. : HRP-60242

Synonyme

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using HRP-60242

  HEK-293 cells were subjected to SDS PAGE followed by western blot with HRP-60242 (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using HRP-60242

  Various lysates were subjected to SDS PAGE followed by western blot with HRP-60242 (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HT-29-Zellen, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

Sample-dependent, check data in validation data gallery

Produktinformation

HRP-60242 bindet in WB OCT4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OCT4 fusion protein Ag1794
• Vollständiger Name: POU class 5 homeobox 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 50-52 kDa
• GenBank-Zugangsnummer: BC020712
• Gene symbol: POU5F1
• Gene ID (NCBI): 5460
• Konjugation: HRP Fluorescent Dye
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).