ODC1 Polyklonaler Antikörper

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• KD/KO Validated

ODC1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 17003-1-AP

Synonyme

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

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Galerie der Validierungsdaten

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  WB analysis of rat thymus using 17003-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 17003-1-AP (ODC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 17003-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17003-1-AP (ODC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17003-1-AP (ODC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17003-1-AP (ODC1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 17003-1-AP

  Immunofluorescent analysis of HepG2 cells, using ODC1 antibody 17003-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Thymusgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe, humanes Plazenta-Gewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Produktinformation

17003-1-AP bindet in WB, IHC, IF, ELISA ODC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ODC1 fusion protein Ag10699
• Vollständiger Name: ornithine decarboxylase 1
• Berechnetes Molekulargewicht: 461 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC025296
• Gene symbol: ODC1
• Gene ID (NCBI): 4953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.

Publikationen

Species	Application	Title
human	IHC	NPJ Precis Oncol; Integrated multiomics analysis of hepatoblastoma unravels its heterogeneity and provides novel druggable targets; Authors - Masahiro Sekiguchi
human	IHC	NPJ Precis Oncol; Integrated multiomics analysis of hepatoblastoma unravels its heterogeneity and provides novel druggable targets.; Authors - Masahiro Sekiguchi ; KD Validated
mouse	WB	Am J Physiol Cell Physiol; The Regulation of Polyamine Pathway Proteins in Models of Skeletal Muscle Hypertrophy and Atrophy - a potential role for mTORC1.; Authors - Michael Tabbaa
human	WB	Life Sci; Ornithine is a key mediator in hyperphosphatemia-mediated human umbilical vein endothelial cell apoptosis: Insights gained from metabolomics.; Authors - Rong Zhou
human	WB	Arch Pharm Res; Spermidine rescues proximal tubular cells from oxidative stress and necrosis after ischemic acute kidney injury.; Authors - Jinu Kim ; KO Validated
mouse	WB	Anat Cell Biol; PMID: 30310711; Authors - Sang Pil Yoon ; KD Validated