ODC1 Monoklonaler Antikörper

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ODC1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A1E3

Kat-Nr. : 67336-1-PBS

Synonyme

2A1E3, EC:4.1.1.17, ODC

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  WB analysis of MCF-7 using 67336-1-Ig (same clone as 67336-1-PBS)

  WB result of ODC1 antibody (67336-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ODC1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67336-1-Ig (same clone as 67336-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67336-1-Ig (same clone as 67336-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 67336-1-Ig (same clone as 67336-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67336-1-Ig (same clone as 67336-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ODC1 antibody (67336-1-Ig, Clone: 2A1E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67336-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67336-1-PBS bindet in WB, IF/ICC, Indirect ELISA ODC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ODC1 fusion protein Ag10927
• Vollständiger Name: ornithine decarboxylase 1
• Berechnetes Molekulargewicht: 461 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC025296
• Gene symbol: ODC1
• Gene ID (NCBI): 4953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.