ODF2 Rekombinanter Antikörper

ODF2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251271E12

Kat-Nr. : 86459-2-RR

Synonyme

Cenexin, ODF2/1

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  WB analysis using 86459-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86459-2-RR (ODF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86459-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86459-2-RR against Human ODF2 were performed. The affinity constant is 0.153 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: OVCAR-3 cells, Jurkat-Zellen, MCF-7-Zellen, Mauslungengewebe, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86459-2-RR bindet in WB, ELISA ODF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ODF2 fusion protein Ag2693
• Vollständiger Name: outer dense fiber of sperm tails 2
• Berechnetes Molekulargewicht: 763 aa, 89 kDa
• Beobachtetes Molekulargewicht: 80-100 kDa
• GenBank-Zugangsnummer: BC010629
• Gene symbol: ODF2
• Gene ID (NCBI): 4957
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.