Teneurin 1 Polyklonaler Antikörper

Teneurin 1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21696-1-AP

Synonyme

ODZ1, ODZ3, Protein Odd Oz/ten m homolog 1, Ten 1, TEN M1, Tenascin M1, Teneurin 1, Teneurin-1, TNM, TNM1

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  WB analysis of H69/H146 cells using 21696-1-AP

  WB result of anti-ODZ1 (21696-1-AP) with two cell lines by Dr. Petrini, Iacopo.

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  WB analysis using 21696-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21696-1-AP (ODZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21696-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21696-1-AP (ODZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21696-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21696-1-AP (ODZ1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21696-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21696-1-AP (ODZ1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21696-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21696-1-AP (ODZ1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21696-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21696-1-AP (Teneurin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21696-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21696-1-AP (Teneurin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, H69/H146-Zellen, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

21696-1-AP bindet in WB, IHC, ELISA Teneurin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Teneurin 1 fusion protein Ag16289
• Vollständiger Name: odz, odd Oz/ten-m homolog 1(Drosophila)
• Berechnetes Molekulargewicht: 2732 aa, 306 kDa
• Beobachtetes Molekulargewicht: 305 kDa
• GenBank-Zugangsnummer: BC140783
• Gene symbol: Teneurin 1
• Gene ID (NCBI): 10178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ODZ1 (also known as teneurin-1, TENM1), which plays a key role in central nervous system ontogenesis. ODZ1 is upregulated in Glioblastoma cells through a Stat3-mediated pathway activated by IL-6, which is released by tumor-associated monocytes. In addition, a hypoxic microenvironment regulates GBM tumor cell migration in part by inducing ODZ1 through hypomethylation of a CpG island in the ODZ1 promoter.

Publikationen

Species	Application	Title
human	WB	Nature; The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.; Authors - Barretina Jordi J
human	WB	Genet Res (Camb); Impact of Extracellular Matrix-Related Genes on the Tumor Microenvironment and Prognostic Indicators in Esophageal Cancer: A Comprehensive Analytical Study; Authors - Yinghong Wu