OGDH Monoklonaler Antikörper

OGDH Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H7A2

Kat-Nr. : 66285-1-PBS

Synonyme

AKGDH, E1k, OGDC, OGDC E1, OGDH

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  WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66285-1-Ig (same clone as 66285-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OGDH antibody (66285-1-Ig, Clone: 1H7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66285-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA OGDH und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OGDH fusion protein Ag7484
• Vollständiger Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Berechnetes Molekulargewicht: 116 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC004964
• Gene symbol: OGDH
• Gene ID (NCBI): 4967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.