OGDH Monoklonaler Antikörper

OGDH Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7A2

Kat-Nr. : CL488-66285

Synonyme

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

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  IF Staining of HepG2 using CL488-66285

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 OGDH antibody (CL488-66285, Clone: 1H7A2 ) at dilution of 1:200, CL594-phalloidin (red).

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  IF Staining of L02 using CL488-66285

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite® Plus 488-conjugated OGDH antibody (CL488-66285, Clone: 1H7A2 ) at dilution of 1:100, CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, L02-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66285 bindet in IF/ICC OGDH und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OGDH fusion protein Ag7484
• Vollständiger Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Berechnetes Molekulargewicht: 116 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC004964
• Gene symbol: OGDH
• Gene ID (NCBI): 4967
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.