OGDHL Rekombinanter Antikörper

OGDHL Rekombinant Antikörper für IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230228B2

Kat-Nr. : 82981-1-PBS

Synonyme

230228B2, 2-oxoglutarate dehydrogenase complex component E1-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, Alpha-ketoglutarate dehydrogenase-like, EC:1.2.4.2

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  IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

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  FC experiment of Caco-2 using 82981-1-RR (same clone as 82981-1-PBS)

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug OGDHL Recombinant antibody (82981-1-RR, Clone:230228B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82981-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82981-1-RR against Human OGDHL were performed. The affinity constant is 4.98 nM.

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Geprüfte Anwendungen

Produktinformation

82981-1-PBS bindet in IHC, FC (Intra), Indirect ELISA OGDHL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OGDHL fusion protein Ag10794
• Vollständiger Name: oxoglutarate dehydrogenase-like
• Berechnetes Molekulargewicht: 1010 aa, 115 kDa
• GenBank-Zugangsnummer: BC026320
• Gene symbol: OGDHL
• Gene ID (NCBI): 55753
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.