OGFR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OGFR Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 11177-1-AP

Synonyme

OGFR, opioid growth factor receptor, Protein 7 60, Zeta type opioid receptor

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 11177-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11177-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11177-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11177-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 11177-1-AP

  IP Result of anti-OGFR (IP:11177-1-AP, 5ug; Detection:11177-1-AP 1:500) with COLO 320 cells lysate 1200ug.

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  IHC staining of human colon cancer using 11177-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11177-1-AP (OGFR antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of COLO 320 using 11177-1-AP

  Immunofluorescent analysis of COLO 320 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of L02 using 11177-1-AP

  Immunofluorescent analysis of L02 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 11177-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug OGFR antibody (11177-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A549-Zellen, HEK-293-Zellen, L02-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: COLO 320-Zellen, L02-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 10 publications below
• IF: See 1 publications below

Produktinformation

11177-1-AP bindet in WB, IP, IHC, IF, FC, ELISA OGFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OGFR fusion protein Ag1671
• Vollständiger Name: opioid growth factor receptor
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC014137
• Gene symbol: OGFR
• Gene ID (NCBI): 11054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Int J Biol Sci; CIRBP-OGFR axis safeguards against cardiomyocyte apoptosis and cardiotoxicity induced by chemotherapy.; Authors - Cihang Liu ; KD Validated
human	IHC	Front Oncol; Opioid Receptor Expression in Colorectal Cancer: A Nested Matched Case-Control Study.; Authors - Amparo Belltall
human	IHC	Int Immunopharmacol; Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor.; Authors - Na Qu
human	WB, IHC	Int Immunopharmacol; Methionine enkephalin activates autophagy and stimulates tumour cell immunogenicity in human cutaneous squamous cell carcinoma.; Authors - Xueli Bai
human	WB, IHC, IF	Int Immunopharmacol; Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma.; Authors - Xueli Bai
human	WB,IHC	Int Immunopharmacol; A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment.; Authors - Shuling Zhang ; KD Validated