OGFR Rekombinanter Antikörper

OGFR Rekombinant Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250452E7

Kat-Nr. : 85998-1-PBS

Synonyme

Opioid growth factor receptor, Protein 7 60, Protein 7-60, Zeta type opioid receptor, Zeta-type opioid receptor

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  WB analysis using 85998-1-RR (same clone as 85998-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85998-1-RR (OGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

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  IP experiment of COLO 320 using 85998-1-RR (same clone as 85998-1-PBS)

  IP result of anti-OGFR (IP:85998-1-RR, 4ug; Detection:85998-1-RR 1:3000) with COLO 320 cells lysate 1920 ug. This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85998-1-RR (same clone as 85998-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85998-1-RR (OGFR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85998-1-RR (same clone as 85998-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85998-1-RR (OGFR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 85998-1-RR (same clone as 85998-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using OGFR antibody (85998-1-RR, Clone: 250452E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85998-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85998-1-RR against Human OGFR were performed. The affinity constant is 0.211 nM.

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Geprüfte Anwendungen

Produktinformation

85998-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA OGFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OGFR fusion protein Ag1671
• Vollständiger Name: opioid growth factor receptor
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC014137
• Gene symbol: OGFR
• Gene ID (NCBI): 11054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.