OGG1 Polyklonaler Antikörper

OGG1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Rezensionen (3)

Kat-Nr. : 15125-1-AP

Synonyme

8 oxoguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase, Ogg 1

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  WB analysis using 15125-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15125-1-AP (OGG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 15125-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15125-1-AP (OGG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15125-1-AP

  IP result of anti-OGG1 (IP:15125-1-AP, 4ug; Detection:15125-1-AP 1:500) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of mouse liver using 15125-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15125-1-AP (OGG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15125-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15125-1-AP (OGG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 15125-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using OGG1 antibody (15125-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 31 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

15125-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA OGG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OGG1 fusion protein Ag7204
• Vollständiger Name: 8-oxoguanine DNA glycosylase
• Berechnetes Molekulargewicht: 22 kDa, 36-40 kDa, 45-57 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa, 45-50 kDa
• GenBank-Zugangsnummer: BC000657
• Gene symbol: OGG1
• Gene ID (NCBI): 4968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base by product that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa). Our OGG1 antibody detects all the isoforms. We always got the strongest 47 kDa corresponds to isoform Beta in our detection and some weaker bands (with long time exposure). The expression amount of Beta is higher than other isoforms from our data. This antibody has been cited in more than 4 publications, WB and IHC detection in mouse and human.

Publikationen

Species	Application	Title
mouse	IHC	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
human	WB	Redox Biol; Oxidative damage to lung mitochondrial DNA is a key contributor to the development of chemical lung injury; Authors - Shubham Dubey
mouse	WB	PLoS Genet; Heterozygosity for a hypomorphic polβ mutation reduces the expansion frequency in a mouse model of the fragile x-related disorders.; Authors - Rachel Adihe Lokanga
mouse	WB	JHEP Rep; TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signalling; Authors - Wantong Su
mouse	WB	Phytomedicine; Nrf2-dependent hepatoprotective effect of ellagic acid in titanium dioxide nanoparticles-induced liver injury; Authors - Kunpeng Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shubhangi (Verified Customer) (07-05-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: Ac16

OGG1 Antibody Western Blot validation (1000 dilution) in Ac16 (Cat no:15125-1-AP)

FH

s (Verified Customer) (12-12-2022)

very good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mouse lung

OGG1 Antibody Western Blot validation (1000 dilution) in mouse lung (Cat no:15125-1-AP)

FH

Ruyi (Verified Customer) (08-19-2019)

This antibody works well in my WB experiments.

• Applications: Western Blot,