OGG1 Rekombinanter Antikörper

OGG1 Rekombinant Antikörper für IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240940D4

Kat-Nr. : 83973-4-PBS

Synonyme

Ogg 1, N-glycosylase/DNA lyase, DNA-(apurinic or apyrimidinic site) lyase, 8-oxoguanine DNA glycosylase, 8 oxoguanine DNA glycosylase

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  IF Staining of HepG2 using 83973-4-RR (same clone as 83973-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGG1 antibody (83973-4-RR, Clone: 240940D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83973-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83973-4-RR (same clone as 83973-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ogg1 Recombinant Antibody (83973-4-RR, Clone:240940D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83973-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83973-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83973-4-RR against Human OGG1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83973-4-PBS bindet in IF/ICC, FC (Intra), Indirect ELISA OGG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OGG1 fusion protein Ag7204
• Vollständiger Name: 8-oxoguanine DNA glycosylase
• Berechnetes Molekulargewicht: 22 kDa, 36-40 kDa, 45-57 kDa
• GenBank-Zugangsnummer: BC000657
• Gene symbol: OGG1
• Gene ID (NCBI): 4968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa).