OGG1 Rekombinanter Antikörper

OGG1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240940H3

Kat-Nr. : 83973-5-PBS

Synonyme

240940H3, 8 oxoguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase

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  WB analysis using 83973-5-RR (same clone as 83973-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83973-5-RR (OGG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83973-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83973-5-RR (same clone as 83973-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using OGG1 antibody (83973-5-RR, Clone: 240940H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83973-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83973-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83973-5-RR against Human OGG1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83973-5-PBS bindet in WB, IF/ICC, Indirect ELISA OGG1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OGG1 fusion protein Ag7204
• Vollständiger Name: 8-oxoguanine DNA glycosylase
• Berechnetes Molekulargewicht: 22 kDa, 36-40 kDa, 45-57 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC000657
• Gene symbol: OGG1
• Gene ID (NCBI): 4968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base by product that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa).