Osteoglycin Polyklonaler Antikörper

Osteoglycin Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 12755-1-AP

Synonyme

Mimecan, OG, OGN, OIF, Osteoglycin, Osteoinductive factor, SLRR3A

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 12755-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12755-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 12755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12755-1-AP

  Immunofluorescent analysis of HepG2 cells, using OGN antibody 12755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

12755-1-AP bindet in WB, IHC, IF, ELISA Osteoglycin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Osteoglycin fusion protein Ag3484
• Vollständiger Name: osteoglycin
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC037273
• Gene symbol: OGN
• Gene ID (NCBI): 4969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

Publikationen

Species	Application	Title
human	IHC, IF	FASEB J; The "Matrisome" reveals the characterization of skin keloid microenvironment.; Authors - Shikun Zhang
mouse	IHC	J Inflamm Res; The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome; Authors - Haishi Zheng
mouse	IHC	Sci Rep; Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.; Authors - Zhiping Cao
human	WB	Onco Targets Ther; Osteoglycin (OGN) Inhibits Cell Proliferation and Invasiveness in Breast Cancer via PI3K/Akt/mTOR Signaling Pathway.; Authors - Tao Xu
human	WB	Front Immunol; Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics; Authors - Wei Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Huai-Chin (Verified Customer) (10-24-2022)

Multiple bands in Western blot. There are two dominant bands at ~40KD and ~55KD. The one ~40KD maybe the band of interest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: used on both mouse and human cell lines