Osteoglycin Monoklonaler Antikörper

Osteoglycin Monoklonal Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

2A5F11

Kat-Nr. : 66382-1-PBS

Synonyme

OGN, Osteoinductive factor, OIF, OG, Mimecan

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  WB analysis of human artery using 66382-1-Ig (same clone as 66382-1-PBS)

  Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  WB analysis of pig artery using 66382-1-Ig (same clone as 66382-1-PBS)

  Pig artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50723-1

  Sandwich ELISA standard curve of MP50723-1, Human Osteoglycin Monoclonal Matched Antibody Pair - PBS only. 66382-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3484. 66382-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP50723-1

  Plasma of six individual healthy human donors was measured. The Osteoglycin concentration of detected samples was determined to be 1.7 ng/mL with a range of 1.2-2.2 ng/mL.

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  Sample test of MP50723-1

  Serum of six individual healthy human donors was measured. The Osteoglycin concentration of detected samples was determined to be 1.9 ng/mL with a range of 1.4-2.3 ng/mL.

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  IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50723-1

  Cytometric bead array standard curve of MP50723-1, OGN/Osteoglycin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66382-1-PBS. Detection antibody: 66382-2-PBS. Standard:Ag3484. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66382-1-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Osteoglycin und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Osteoglycin fusion protein Ag3484
• Vollständiger Name: osteoglycin
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC037273
• Gene symbol: OGN
• Gene ID (NCBI): 4969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.