OGT Polyklonaler Antikörper

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• KD/KO Validated

OGT Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, IP-MS, ELISA

Konjugation

Unkonjugiert

Publikationen(76)

Rezensionen (3)

Kat-Nr. : 11576-2-AP

Synonyme

EC:2.4.1.255, HRNT1, O GLCNAC, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit

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  WB analysis using 11576-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11576-2-AP (OGT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11576-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP (OGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11576-2-AP

  IP result of anti-OGT (IP:11576-2-AP, 3ug; Detection:11576-2-AP 1:1000) with mouse brain tissue lysate 8000ug.

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  IHC staining of human colon cancer using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11576-2-AP (OGT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Pankreaskarzinomgewebe, Maushirngewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 66 publications below
• IHC: See 15 publications below
• IF: See 10 publications below
• IP: See 9 publications below
• CoIP: See 5 publications below

Produktinformation

11576-2-AP bindet in WB, IHC, IF, IP, CoIP, IP-MS, ELISA OGT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OGT fusion protein Ag2160
• Vollständiger Name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• Berechnetes Molekulargewicht: 1046 aa, 117 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC014434
• Gene symbol: OGT
• Gene ID (NCBI): 8473
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

Publikationen

Species	Application	Title
mouse,human	WB,IF,CoIP	Cell Metab; Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction; Authors - Yuki Tatekoshi
human	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
mouse	WB	Nat Metab; Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C; Authors - Yang Li
human	WB	J Clin Invest; Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation; Authors - Di Cui
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Nat Chem Biol; Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase.; Authors - Chia-Wei Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christelle (Verified Customer) (01-13-2025)

Immunofluorescent analysis of (PFA 4%) fixed human renal mesangial cells using OGT antibody (11576-2-AP) at dilution of 1:50 and AF488-Conjugated Donkey Anti-Rabbit IgG (Life Technologies), Star Red membrane probe (Abberior, yellow) and DAPI (blue). 2 files uploaded : 1 aquisition without the membrane probe and the same aquisition with the membrane probe

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human Renal Mesangial Cells (HRMC)

OGT Antibody Immunofluorescence validation (1:50 dilution) in Human Renal Mesangial Cells (HRMC) (Cat no:11576-2-AP)

FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works well for WB, dilution 1:1000

• Applications: Western Blot
• Cell Tissue Type: Human pulmonary artery smooth muscle cells

FH

Isha (Verified Customer) (05-18-2022)

Antibody worked perfect at 1:1000 dilution for W.B. and 1:500 for IHC

• Applications: Western Blot, Immunohistochemistry