OGT Monoklonaler Antikörper

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OGT Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B2A6

Kat-Nr. : 66823-1-PBS

Synonyme

FLJ23071, HRNT1, O GLCNAC, OGT

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  WB analysis using 66823-1-Ig (same clone as 66823-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66823-1-Ig (OGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGT antibody (66823-1-Ig, Clone: 2B2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66823-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA OGT und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OGT fusion protein Ag28402
• Vollständiger Name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• Berechnetes Molekulargewicht: 1046 aa, 117 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC014434
• Gene symbol: OGT
• Gene ID (NCBI): 8473
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.