OIP5 Polyklonaler Antikörper

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• KD/KO Validated

OIP5 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 12142-1-AP

Synonyme

5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta

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Galerie der Validierungsdaten

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  WB analysis using 12142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12142-1-AP (OIP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12142-1-AP

  IP Result of anti-OIP5 (IP:12142-1-AP, 3ug; Detection:12142-1-AP 1:500) with Jurkat cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

12142-1-AP bindet in WB, IP, IHC, IF, ELISA OIP5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OIP5 fusion protein Ag2790
• Vollständiger Name: Opa interacting protein 5
• Berechnetes Molekulargewicht: 229 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC015050
• Gene symbol: OIP5
• Gene ID (NCBI): 11339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OIP5, also named as IS18B and CT86, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It is over expressed in the majority of primary lung and esophageal cancers. OIP5 is a promising target for developing new prognostic biomarkers and anti-cancer drugs.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Sci Bull (Beijing); Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer; Authors - Binbin Zheng
human	WB	Neuro Oncol; Cancer-testis specific gene OIP5: a downstream gene of E2F1 that promotes tumorigenesis and metastasis in glioblastoma by stabilizing E2F1 signaling.; Authors - Jiang He ; KD Validated
human	WB	Oncotarget; OIP5, a target of miR-15b-5p, regulates hepatocellular carcinoma growth and metastasis through the AKT/mTORC1 and β-catenin signaling pathways.; Authors - Hua Li
human	WB	J Biol Chem; Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.; Authors - Jianyu Wang ; KD Validated
human	WB,IHC,IF	Int J Oncol; Characterization of Opa interacting protein 5 as a new biomarker and therapeutic target for oral cancer.; Authors - Ming Zhu ; KD Validated
human	WB,IHC,IF	Cancer Sci; Characterization of an Opa interacting protein 5 involved in lung and esophageal carcinogenesis.; Authors - Koinuma Junkichi J

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