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OIP5 Rekombinanter Antikörper

OIP5 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250073A7

Kat-Nr. : 85757-5-PBS

Synonyme

CT86, LINT 25, LINT25, MIS18B, OIP 5

Filter:
  • Western Blot (WB) analysis of various lysates using OIP5 Recombinant antibody (85757-5-RR)

    WB analysis using 85757-5-RR (same clone as 85757-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85757-5-RR (OIP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85757-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85757-5-RR

    Affinity and Kinetic Characterization of 85757-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85757-5-RR against Human OIP5 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85757-5-PBS bindet in WB, Indirect ELISA OIP5 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen OIP5 fusion protein Ag2790
Vollständiger Name Opa interacting protein 5
Berechnetes Molekulargewicht 229 aa, 25 kDa
Beobachtetes Molekulargewicht 25-30 kDa
GenBank-ZugangsnummerBC015050
Gene symbol OIP5
Gene ID (NCBI) 11339
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OIP5 is also known as Mis18β. Vertebrates possess two Mis18 paralogs, Mis18α and Mis18β. Mis18 has been previously shown to affect histone modifications and the methylation status of underlying chromatin . Eliminating histone methylation within the alpha satellite DNA repeats of a human artificial chromosome alters the recruitment of HJURP .The Mis18core complex was originally characterized as an oligomer with a proposed 2:2 stoichiometry of Mis18α and Mis18β, but additional work demonstrated a 4:2 Mis18α:Mis18β hexamer. (PMID: 31492860, PMID: 26942680)