OLFML1 Rekombinanter Antikörper

OLFML1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241561G7

Kat-Nr. : 84454-1-RR

Synonyme

UNQ564, olfactomedin like 1, 241561G7

---

• 

  WB analysis using 84454-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84454-1-RR (OLFML1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84454-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84454-1-RR against Human OLFML1 were performed. The affinity constant is 0.807 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HuH-7-Zellen, Mauslebergewebe, Maushodengewebe, Rattenlebergewebe, Rattenhodengewebe, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84454-1-RR bindet in WB, ELISA OLFML1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OLFML1 fusion protein Ag35125
• Vollständiger Name: olfactomedin-like 1
• Berechnetes Molekulargewicht: 402 aa, 46 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC092488
• Gene symbol: OLFML1
• Gene ID (NCBI): 283298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OLFML1 (Olfactomedin-like protein 1) is a novel member of human OLF protein family with an olfectamine domain (143-397 aa) in its C-terminus. OLFML1 exhibits the promotion of cell proliferation, along with the increase of percentage of S phase cells. OLFML1 is considered a secreted glycoprotein that enhances cell cycle progression in human cancer cell lines in vitro (PMID: 30266405). OLFML1 contains three potential N-glycosylation sites (residues 66, 138 and 183) and could be modified by the N-glycosylation (PMID: 18708057).