OLIG1 Polyklonaler Antikörper

OLIG1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26763-1-PBS

Synonyme

Oligo1, Class E basic helix-loop-helix protein 21, Class B basic helix-loop-helix protein 6, bHLHe21, bHLHb6

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  WB analysis using 26763-1-AP (same clone as 26763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26763-1-AP (OLIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26763-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 26763-1-AP (same clone as 26763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26763-1-AP (OLIG1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26763-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 26763-1-AP (same clone as 26763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26763-1-AP (OLIG1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26763-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26763-1-PBS bindet in WB, Indirect ELISA OLIG1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OLIG1 fusion protein Ag25128
• Vollständiger Name: oligodendrocyte transcription factor 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC026989
• Gene symbol: OLIG1
• Gene ID (NCBI): 116448
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors includes OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.