OLIG2 Polyklonaler Antikörper

OLIG2 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13999-1-PBS

Synonyme

5O7, bHLHb1, bHLHe19, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19

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  WB analysis using 13999-1-AP (same clone as 13999-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13999-1-AP (OLIG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 13999-1-AP (same clone as 13999-1-PBS)

  IP result of anti-OLIG2 (IP:13999-1-AP, 4ug; Detection:13999-1-AP 1:1000) with mouse brain tissue lysate 1600 ug. This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 13999-1-AP (same clone as 13999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13999-1-AP (OLIG2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13999-1-AP (same clone as 13999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13999-1-AP (OLIG2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13999-1-AP (same clone as 13999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13999-1-AP (OLIG2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13999-1-AP (same clone as 13999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13999-1-AP (OLIG2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13999-1-AP (same clone as 13999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13999-1-AP (OLIG2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 13999-1-AP (same clone as 13999-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using OLIG2 antibody (13999-1-AP) at dilution of 1:400 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), NeuN antibody (66836-1-Ig, Clone: 3A4C1, Magenta), CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 13999-1-AP (same clone as 13999-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using OLIG2 antibody (13999-1-AP) at dilution of 1:400 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), NeuN antibody (66836-1-Ig, Clone: 3A4C1, Magenta), CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 13999-1-AP (same clone as 13999-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using OLIG2 antibody (13999-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 13999-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13999-1-PBS bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA OLIG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OLIG2 fusion protein Ag5089
• Vollständiger Name: oligodendrocyte lineage transcription factor 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32-36 kDa
• GenBank-Zugangsnummer: BC047511
• Gene symbol: OLIG2
• Gene ID (NCBI): 10215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the specificity of Olig2?

Oligodendrocyte transcription factor 2 (OLIG2) is expressed by cells found in the central nervous system (CNS) called oligodendrocyte precursor cells (OPCs), which form the myelin sheaths wrapping the axons of neurons in the brain and spinal cord. OPCs differentiate into oligodendrocytes that form the myelin, providing metabolic support and saltatory conduction. OLIG2 is expressed broadly throughout their development to OPCs. This protein can be used to identify many cells of the oligodendrocyte lineage. It is found mostly in the nucleoplasm but also in the cytoplasm.

What is the function of OLIG2?

The basic helix-loop-helix structure of OLIG2 allows it to function as a transcription factor, determining cell fate in the development of neural tissue, where it is located at the pMN domain in the embryonic spinal cord. Expression of OLIG2 causes neural precursors to develop into oligodendrocytes or into motor neurons and expression is then maintained postnatally. OLIG2 co-operates with other factors to cause this differentiation from precursors, although overexpression alone can cause differentiation to the oligodendrocyte lineage.¹ The continued expression of OLIG2 in OPCs indicates an ongoing role in the maintenance of their stemness.

What is the involvement of OLIG2 in disease?

The expression of OLIG2 in glioblastoma, the most common type of malignant brain tumor in adults, is well characterized, where the pathological function is an extension of the normal function. Stem-like cells that propagate the tumor growth have been shown to be OLIG2-expressing, and are one of the key transcription factors involved in the re-programming of differentiated cells of the tumor to stem-like cells.² OLIG2 has also been associated with demyelinating diseases like multiple sclerosis (MS), as OPCs have been shown to be involved in the process of remyelination.³

1.     Liu, Z. et al. Induction of oligodendrocyte differentiation by Olig2 and Sox10: Evidence for reciprocal interactions and dosage-dependent mechanisms. Dev. Biol. 302, 683-693 (2007).

2.     Wegener, A. et al. Gain of Olig2 function in oligodendrocyte progenitors promotes remyelination. Brain 138, 120-35 (2015).

3.     Ettle, B., Schlachetzki, J. C. M. & Winkler, J. Oligodendroglia and Myelin in Neurodegenerative Diseases: More Than Just Bystanders? Mol. Neurobiol. 53, 3046-3062 (2016).