OLIG2 Polyklonaler Antikörper

OLIG2 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25754-1-PBS

Synonyme

OLIG 2, Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1

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  WB analysis of rat brain using 25754-1-AP (same clone as 25754-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25754-1-AP (OLIG2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 25754-1-AP (same clone as 25754-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 25754-1-AP (OLIG2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (25754-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (25754-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25754-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA OLIG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OLIG2 fusion protein Ag22642
• Vollständiger Name: oligodendrocyte lineage transcription factor 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC047511
• Gene symbol: OLIG2
• Gene ID (NCBI): 10215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.