OLIG2 (for WB only) Monoklonaler Antikörper

OLIG2 (for WB only) Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

CloneNo.

1E8C5

Kat-Nr. : 66513-1-Ig

Synonyme

OLIG2, 1E8C5, 5O7, bHLHb1, bHLHe19

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  WB analysis of human brain using 66513-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 66513-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below

Produktinformation

66513-1-Ig bindet in WB, ELISA OLIG2 (for WB only) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OLIG2 (for WB only) fusion protein Ag18838
• Vollständiger Name: oligodendrocyte lineage transcription factor 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 35-39 kDa
• GenBank-Zugangsnummer: BC047511
• Gene symbol: OLIG2
• Gene ID (NCBI): 10215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

Publikationen

Species	Application	Title
		Cell Metab; Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK; Authors - Yajuan Li
human	WB	Theranostics; Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2; Authors - Zirong Chen
human	WB	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
	WB	Int Immunopharmacol; Baicalin and kaempferol alleviates cuprizone-induced demyelination and microglial activation by inhibiting the STAT3 and NF-κB signaling pathways; Authors - Liming Liu
human	WB	Cell Biochem Funct; Ring finger protein 216 loss-of-function induces white matter hyperintensities by inhibiting oligodendroglia proliferation; Authors - Wenqing Xu
		Phytother Res; Celastrol Mediated Regulation of the HnRNPA1-Thyroxine Axis in the Amygdala Alleviates High Fat Diet-Induced Demyelination and Cognitive Deficits in Mice; Authors - Xuemin Yao