OLR1/LOX1 Rekombinanter Antikörper

OLR1/LOX1 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250525B12

Kat-Nr. : 98524-3-RR

Synonyme

OLR1, CLEC8A, C-type lectin domain family 8 member A, hLOX 1, hLOX1

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  FC experiment of human PBMCs using 98524-3-RR

  1x10^6 untreated or GM-CSF-treated human PBMCs were surface stained with 0.25 ug Anti-Human OLR1/LOX1 Rabbit RecAb (98524-3-RR, Clone: 250525B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 98524-3-RR

  1x10^6 GM-CSF-treated human PBMCs were surface stained with 0.25 ug Anti-Human OLR1/LOX1 Rabbit RecAb (98524-3-RR, Clone: 250525B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human OLR1/LOX1 Rabbit RecAb (98524-3-RR, Clone: 250525B12) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: GM-CSF treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98524-3-RR bindet in FC OLR1/LOX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OLR1/LOX1 fusion protein Eg2911
• Vollständiger Name: oxidized low density lipoprotein (lectin-like) receptor 1
• Berechnetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: NM_002543.3
• Gene symbol: OLR1
• Gene ID (NCBI): 4973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

OLR1/LOX-1 acts as a receptor, in the form of homodimer, that mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein (oxLDL) (PMID: 9052782, 15695803). ORL1 is predominantly expressed in endothelial cells and vascular-rich organs such as the placenta, lung, liver, and brain (PMID: 9828121). OLR1 is a protein containing 273 amino acids with a calculated molecular mass of 31 kDa but an apparent molecular mass of 50 kDa, which may result from the glycosylation of four potential N-linked glycosylation sites located at the C-terminal domain (PMID: 9052782).