OMA1 Monoklonaler Antikörper

OMA1 Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H4D12

Kat-Nr. : 67449-1-PBS

Synonyme

Overlapping with the m-AAA protease 1 homolog, MPRP-1, MPRP1, Metalloprotease-related protein 1, EC:3.4.24.-

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  WB analysis of NIH/3T3 using 67449-1-Ig (same clone as 67449-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  WB analysis using 67449-1-Ig (same clone as 67449-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67449-1-Ig (same clone as 67449-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67449-1-Ig (same clone as 67449-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67449-1-Ig (same clone as 67449-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50571-3

  Cytometric bead array standard curve of MP50571-3, OMA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66572-1-PBS. Detection antibody: 67449-1-PBS. Standard:Ag11201. Range: 3.125-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

67449-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA OMA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OMA1 fusion protein Ag11201
• Vollständiger Name: OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
• Berechnetes Molekulargewicht: 524 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC012915
• Gene symbol: OMA1
• Gene ID (NCBI): 115209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OMA1(Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1(Metalloprotease-related protein 1) and belongs to the peptidase M48 family.It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants,and the 40 kDa form of OMA1 may be the inactive form, with the 60 kDa form being the active enzyme(PMID:20334834)