ZNF830 Polyklonaler Antikörper

ZNF830 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (3)

Kat-Nr. : 25618-1-AP

Synonyme

CCDC16, Coiled-coil domain-containing protein 16, OMCG1, SEL13, Zinc finger protein 830

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  WB analysis using 25618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25618-1-AP (ZNF830 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25618-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25618-1-AP (ZNF830 antibody at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25618-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25618-1-AP (ZNF830 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25618-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25618-1-AP (ZNF830 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25618-1-AP bindet in WB, IHC, ELISA ZNF830 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF830 fusion protein Ag22538
• Vollständiger Name: zinc finger protein 830
• Berechnetes Molekulargewicht: 372 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC011584
• Gene symbol: ZNF830
• Gene ID (NCBI): 91603
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Antibody detect a major band at ~50 kDa with some weaker unspecific bands. Incubation was done overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

ZNF830 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:25618-1-AP)

FH

Tsimafei (Verified Customer) (11-19-2024)

Overall antibody is great and detect a band ~ 50 kDa, though some weak additional bands are visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

ZNF830 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:25618-1-AP)

FH

Moritz (Verified Customer) (08-16-2024)

Ab worked very well! WB, 1:5000, 1h@RT

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T lysate