ONECUT2 Polyklonaler Antikörper

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ONECUT2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21916-1-PBS

Synonyme

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  WB analysis using 21916-1-AP (same clone as 21916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21916-1-AP (same clone as 21916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 21916-1-AP (same clone as 21916-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 21916-1-AP (same clone as 21916-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 21916-1-AP (same clone as 21916-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 21916-1-AP (same clone as 21916-1-PBS)

  IP result of anti-ONECUT2 (IP:21916-1-AP, 4ug; Detection:21916-1-AP 1:300) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 21916-1-AP (same clone as 21916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21916-1-AP (ONECUT2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21916-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21916-1-PBS bindet in WB, IHC, IP, Indirect ELISA ONECUT2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ONECUT2 fusion protein Ag16162
• Vollständiger Name: one cut homeobox 2
• Berechnetes Molekulargewicht: 504 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC108925
• Gene symbol: ONECUT2
• Gene ID (NCBI): 9480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ONECUT2, also named as One cut domain family member 2 or Hepatocyte nuclear factor 6-beta, is a 504 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT2 belongs to the CUT homeobox family and activates the transcription of a number of liver genes such as HNF3B. ONECUT2 is required for liver differnetiation and metabolism. ONECUT2 and ONECUT3 play redundant roles with ONECUT1 in pancreas development.