OPA1 Polyklonaler Antikörper

OPA1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32465-1-AP

Synonyme

OPA1 gene, OPA 1, EC:3.6.5.5, Dynamin-like GTPase OPA1, short form, Dynamin-like GTPase OPA1, mitochondrial

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  WB analysis using 32465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32465-1-AP (OPA1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 32465-1-AP

  IP result of anti-OPA1 (IP:32465-1-AP, 4ug; Detection:32465-1-AP 1:40000) with mouse brain tissue lysate 1840 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32465-1-AP bindet in WB, IP, ELISA OPA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OPA1 fusion protein Ag38295
• Vollständiger Name: optic atrophy 1 (autosomal dominant)
• Berechnetes Molekulargewicht: 960 aa, 112 kDa
• Beobachtetes Molekulargewicht: 80-100 kDa
• GenBank-Zugangsnummer: BC075805
• Gene symbol: OPA1
• Gene ID (NCBI): 4976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms.