OPA3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OPA3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15638-1-AP

Synonyme

MGA3, OPA3, Optic atrophy 3 protein

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Galerie der Validierungsdaten

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  WB analysis using 15638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15638-1-AP (OPA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 15638-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15638-1-AP (OPA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15638-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15638-1-AP (OPA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15638-1-AP (OPA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15638-1-AP

  IP result of anti-OPA3 (IP:15638-1-AP, 4ug; Detection:15638-1-AP 1:1000) with HeLa cells lysate 1240 ug.

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  IHC staining of human liver using 15638-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15638-1-AP (OPA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15638-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15638-1-AP (OPA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 15638-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15638-1-AP (OPA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 15638-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15638-1-AP (OPA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15638-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OPA3 antibody (15638-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, HeLa-Zellen, humanes Nierengewebe, Jurkat-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Produktinformation

15638-1-AP bindet in WB, IP, IHC, IF, ELISA OPA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OPA3 fusion protein Ag8103
• Vollständiger Name: optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
• Berechnetes Molekulargewicht: 179 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC005059
• Gene symbol: OPA3
• Gene ID (NCBI): 80207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The OPA3 cDNA encodes a deduced 179-amino acid protein. Northern blot analysis demonstrated a primary transcript of approximately 5.0 kb that was ubiquitously expressed, most prominently in skeletal muscle and kidney. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract.

Publikationen

Species	Application	Title
mouse	IHC	Hum Mol Genet; Disrupted mitochondrial function in the Opa3L122P mouse model for Costeff Syndrome impairs skeletal integrity.; Authors - Alice E Navein
mouse,rat	WB,IP	Cell Signal; Hydrogen sulfide alleviates mitochondrial damage and ferroptosis by regulating OPA3-NFS1 axis in doxorubicin-induced cardiotoxicity; Authors - Yifan Wang
human,mouse	WB,IHC	Invest Ophthalmol Vis Sci; Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III.; Authors - Powell Kate A KA
bovine	WB	Genomics; A nonsense mutation in the optic atrophy 3 gene (OPA3) causes dilated cardiomyopathy in Red Holstein cattle.; Authors - Owczarek-Lipska Marta M
human	WB	Bioengineered; MYB proto-oncogene like 2 promotes hepatocellular carcinoma growth and glycolysis via binding to the Optic atrophy 3 promoter and activating its expression.; Authors - Miao Liu ; KD Validated
human	IHC	Cancers (Basel); Oncogenic K-ras Induces Mitochondrial OPA3 Expression to Promote Energy Metabolism in Pancreatic Cancer Cells.; Authors - Ning Meng