Oligophrenin 1 Polyklonaler Antikörper

Oligophrenin 1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11076-1-AP

Synonyme

MRX60, oligophrenin 1, OPHN1, OPN1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11076-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11076-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11076-1-AP

  IP result of anti-Oligophrenin 1 (IP:11076-1-AP, 4ug; Detection:11076-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of human cerebellum using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11076-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Cerebellum-Gewebe, humanes Retinoblastom-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

11076-1-AP bindet in WB, IP, IHC, IF, ELISA Oligophrenin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Oligophrenin 1 fusion protein Ag1536
• Vollständiger Name: oligophrenin 1
• Berechnetes Molekulargewicht: 802 aa, 92 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC059393
• Gene symbol: OPHN1
• Gene ID (NCBI): 4983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The OPHN1 gene encodes a Rho-GTPase activating protein (RhoGAP), and mutations in OPHN1 are responsible for non-specific X-linked mental retardation (NSMR). OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons.

Publikationen

Species	Application	Title
human	IHC	Ann Surg Oncol; Additive lymph node dissection may be necessary in minute submucosal cancer of the stomach after endoscopic resection.; Authors - Kim Jie-Hyun JH
human	WB	PLoS One; Oligophrenin-1 (OPHN1), a gene involved in X-linked intellectual disability, undergoes RNA editing and alternative splicing during human brain development.; Authors - Sabina Barresi
mouse	WB,IHC	Gene Expr Patterns; Oligophrenin-1 (Ophn1) is expressed in mouse retinal vessels.; Authors - Sel Saadettin S
mouse	WB	Neurosci Lett; Alpha lipoic acid treatment in late middle age improves cognitive function: Proteomic analysis of the protective mechanisms in the hippocampus; Authors - Jian Zhang