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Osteopontin Polyklonaler Antikörper

Osteopontin Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(434)

Kat-Nr. : 22952-1-AP

Synonyme

OPN, BNSP, Bone sialoprotein 1, BSPI, ETA 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Mausnierengewebe
Erfolgreiche Detektion in IHCMausnierengewebe, Mauslungengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, HEK-293-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

22952-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA Osteopontin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Kaninchen, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Osteopontin fusion protein Ag19216
Vollständiger Name secreted phosphoprotein 1
Berechnetes Molekulargewicht 314 aa, 35 kDa
Beobachtetes Molekulargewicht 70 kDa, 44-66 kDa
GenBank-ZugangsnummerBC007016
Gene symbol Osteopontin
Gene ID (NCBI) 6696
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Osteopontin antibody 22952-1-APProtokoll herunterladen
IHC protocol for Osteopontin antibody 22952-1-APProtokoll herunterladenl
IF protocol for Osteopontin antibody 22952-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Circulation

Carbonylation of Runx2 at K176 by 4-Hydroxynonenal Accelerates Vascular Calcification

Authors - Xiaoxuan Zhai
mouseIF

Adv Mater

Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon-Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects

Authors - Congyang Mao
humanIF

Cell Stem Cell

Decoding the temporal and regional specification of microglia in the developing human brain.

Authors - Yanxin Li
humanIF

Nat Aging

Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer

Authors - Yifei Cheng
rat,humanIF

Bioact Mater

Urchin-like multiscale structured fluorinated hydroxyapatite as versatile filler for caries restoration dental resin composites

Authors - Shuning Zhang
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Apoorva (Verified Customer) (06-04-2025)

The antibody worked well with both tissues and cell. I tested the product with both IHC and IF

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Mouse lung tissue/ MEFs
FH

Shinford (Verified Customer) (10-26-2023)

The WB bands exhibit remarkable strength and clarity, with various treatments leading to significant changes in expression.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Lung cancer cell lines and lung cancer tissues with different treatment
Osteopontin Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines and lung cancer tissues with different treatment (Cat no:22952-1-AP)
FH

Shinford (Verified Customer) (09-24-2023)

This antibody works well in esophageal cancer and lung cancer cell lines, and the detection antibody band is clear. It is recommended to use a diluted concentration of 1:2000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cancer cell lines
Osteopontin Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:22952-1-AP)
FH

Elisa (Verified Customer) (08-16-2023)

Tested on mouse skull sections, with appropriate blocking: give a nice stain of osteopontin, but maybe it can be optimized to be able to use an higher dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse skull
FH

Davide (Verified Customer) (09-20-2022)

The antibody works really well. Fantastic product, and the possibility to try the product with the free sample is something really appreciated. Will surely buy a full vial soon.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocytes
Osteopontin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocytes (Cat no:22952-1-AP)
FH

WEI (Verified Customer) (03-08-2022)

Strong specific Bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart
FH

LUNFENG (Verified Customer) (01-27-2020)

VERY GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Works with one specific band and one faint non-specific band. However, can be distinguished by protein size. So works fine in general.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney
FH

Jingwen (Verified Customer) (01-17-2020)

This antibody showed the specific band and staining results in both western blot and immunofluorescence staining. I strongly recommend it.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
  • Cell Tissue Type: Mouse heart and cultured cardiomyocytes.
FH

Barbara (Verified Customer) (09-28-2019)

Fluorescence-based detection of OPN (green) expression in paraffin-embedded human aortic tissue, heat mediated antigen retrival with 10mM citrate buffer (pH 6,0)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: aorta
Osteopontin Antibody Immunohistochemistry, validation (1:150 dilution) in aorta (Cat no:22952-1-AP)